Four　endoglucanases(EG1,　EG2,　EG3　and　EG4)　were　isolated　and　purified　to　electrophoretic　homogeneity　from　leavening　of　Trichoderma　viride　M1　by　a　combination　of　DEAE-650C　anion-exchange　chromatography,　CM-650M　cationic-exchange　chromatography,　Sephacryl　S-200　gel　chromatography　and　Octyl　Sepharose　CL-4B　hydrophobic　chromatography.　The　specific　activity　of　EG1,　EG2,　EG3　and　EG4　with　carboxymethyl　cellulose　were　41.83,　139.96,　117.72　and　126.50　U/mg　respectively.　For　EG1,　EG2,　EG3　and　EG4,　the　relative　molecular　weight　were　25.4,　28.8,　56.3,　60.5　ku,　respectively.　And　the　optimum　temperture　were　50,　45,　55,　60　respectively,　while　those　endoglucanases　had　their　optimum　activity　at　pH　6.0,　5.0,　4.0　and　5.0.　The　values　of　Km　for　the　four　endoglucanases　were　9.51,　5.06,　4.16,　4.86　mg/mL　severally.　The　EG1,　EG3　and　EG4　had　the　relatively　good　stability　during　30-50.　The　EG1,　EG2　and　EG4　were　relatively　stable　within　the　pH　range　of　4.0~6.0.　The　activity　of　EG1,　EG2　and　EG3　were　stimulated　by　5mmol/L　Ca2+,　and　inhibited　by　5　mmol/L　Zn2+,　Mg2+　and　K+,　but　the　activity　of　EG4　was　stimulated　by　5　mmol/L　Zn2+,　Mg2+　and　K+.　©,　2014,　Chinese　Institute　of　Food　Science　and　Technology.　All　right　reserved.