Selective　enrichment　of　glycopeptides　from　complex　biological　samples　is　essential　for　MS-based　glycoproteomics,　but　challenges　still　remain.　In　this　work,　glucose-functionalized　magnetic　mesoporous　nanoparticles　(MMNs),　which　hold　the　attractive　features　of　well-defined　core/shell　structure,　high　specific　surface　area　(324m2/g),　narrow　pore　size　distribution　(2.2nm)　and　high　magnetic　responsivity　(69.1emug-1),　were　synthesized　via　click　chemistry　and　applied　to　enrich　glycopeptides　and　glycans.　Taking　advantages　of　the　size-exclusive　effect　of　mesopore　against　proteins　and　the　hydrophilic　interaction　between　glycans　and　glucose,　the　hydrophilic　MMNs　possessed　high　selectivity　for　glycopeptides　at　the　digested　mixtures　of　horseradish　peroxidase　(HRP),　myoglobin　and　β-casein　at　molar　ratio　of　1:1:10,　large　enrichment　capacity　(as　high　as　250mg/g),　high　sensitivity　(50fmol),　excellent　speed　(5min　for　enrichment)　and　high　recovery　of　glycopeptides　(as　high　as　94.6%).　Additionally,　the　MMNs　exhibited　excellent　performance　in　enrichment　of　N-linked　glycans　from　the　digested　human　serum　that　are　made　up　of　peptides,　large　proteins　and　other　compounds.　These　outstanding　features　will　give　the　hydrophilic　MMNs　high　benefit　for　MS　analysis　of　low-abundance　glycopeptides/glycans.　©　2014　Elsevier　B.V.