A　kind　of　mesoporous　silica　molecular　sieves　Bi-MSU-4　were　synthesized　successfully　using　nonionic　ethoxylated　sorbitan　esters　(Tween-x,　x　=　20,-40,-60,　or-80)　as　structure　directors　respectively　and　TEOS　as　silicate　source　at　room　temperature.　Structures　of　the　synthesized　molecular　sieves　were　characterized　by　X-ray　diffraction　(XRD),　FT-IR,　UV-vis,　N2　absorption/desorption　and　SEM　methods.　The　influence　of　factors　such　as　templates,　mineralizer　and　Si/Bi　molar　ratios　on　Bi-MSU-4　mesoporous　structure　and　styrene　catalytic　oxidation　were　examined.　The　results　indicated　that　Bi-MSU-4　exhibited　mesoporous　structures　and　bismuth　entered　the　framework　already.　The　pore　size　and　pore　volume　of　samples　increased　with　the　augment　of　the　hydrophobic　chain　length　of　templates,　which　led　to　the　rise　of　catalytic　performance.　In　addition　mineralizing　structured　framework　and　expelled　it　from　hydrophilic　PEO　shell.　With　the　increasing　of　Si/Bi　molar　ratio　catalytic　performance　increased　first　and　then　decreased.　T-XBi-MSU-4　were　superior　in　styrene　oxidation　reaction　and　the　conversion　of　styrene　was　55.0%　and　the　yield　of　benzaldehyde　was　41.5%　especially　for　T-60Bi-MSU-4.