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author：

Ma, Xiaoming (Ma, Xiaoming.) [1] | Chen, Zhonghui (Chen, Zhonghui.) [2] | Zhou, Jin (Zhou, Jin.) [3] | Weng, Wen (Weng, Wen.) [4] | Zheng, Ou (Zheng, Ou.) [5] | Lin, Zhenyu (Lin, Zhenyu.) [6] | Guo, Longhua (Guo, Longhua.) [7] | Qiu, Bin (Qiu, Bin.) [8] | Chen, Guonan (Chen, Guonan.) [9]

Indexed by：

EI

Abstract：

A　novel　portable　biosensor　for　sensitive　and　selective　detection　of　platelet-derived　growth　factor-BB　(PDGF-BB)　had　been　developed　based　on　the　specific　recognition　between　aptamer　and　protein　using　a　personal　glucose　meter　(PGM)　as　readout.　In　the　presence　of　PDGF-BB,　the　primary　aptamer　of　PDGF-BB　which　is　bound　to　the　surface　of　streptavidin　magnespheres　paramagnetic　particles　(SA-PMPs)　reacts　quantitatively　with　invertase-functionalized　secondary　aptamer　of　PDGF-BB　to　form　a　stable　complex,　resulting　in　the　attachment　of　invertase　on　the　SA-PMPs.　Subsequently,　the　invertase　catalyzes　the　hydrolysis　of　sucrose　to　produce　a　large　amount　of　glucose　and　quantitative　readout　by　the　PGM.　The　enhanced　signal　of　the　PGM　has　a　relationship　with　the　concentration　of　PDGF-BB　in　the　range　of　1.0×10-14M~3.16×10-12M,　and　the　detection　limit　is　2.9fM.　The　proposed　portable　biosensor　had　been　successfully　applied　to　assay　the　PDGF-BB　in　saliva　samples.　©　2013　Elsevier　B.V.

Keyword：

Bioassay Biosensors Glucose Platelets Proteins

Community：

  • [ 1 ] [Ma, Xiaoming]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 2 ] [Chen, Zhonghui]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 3 ] [Zhou, Jin]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 4 ] [Weng, Wen]Department of Chemistry and Environmental Science, Minnan Normal University, Zhangzhou, Fujian 363000, China
  • [ 5 ] [Zheng, Ou]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 6 ] [Lin, Zhenyu]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 7 ] [Guo, Longhua]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 8 ] [Qiu, Bin]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
  • [ 9 ] [Chen, Guonan]MOE Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China

Reprint 's Address：

Email：


weng_wen@126.com
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Source ：

Biosensors and Bioelectronics

ISSN： 0956-5663

Year： 2014

Volume： 55

Page： 412-416

6 . 4 0 9

JCR@2014

1 0 . 7 0 0

JCR@2023

ESI HC Threshold：268

JCR Journal Grade：1

CAS Journal Grade：1

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 49

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

Affiliated Colleges：

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