Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis-inductively coupled plasma mass spectrometry - Details

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Abstract：

In　this　study,　an　ultra-sensitive　method　for　the　quantification　of　lysozyme　based　on　the　Gd3+　diethylenetriamine-N,N,N＇,N″,N″-pentaacetic　acid　labeling　and　capillary　electrophoresis-inductively　coupled　plasma　mass　spectrometry　(CE-ICP-MS)　was　described.　The　Gd3+-tagged　lysozyme　was　effectively　separated　by　capillary　electrophoresis　(CE)　and　sensitively　determined　by　inductively　coupled　plasma　mass　spectrometry　(ICP-MS).　Based　on　the　gadolinium-tagging　and　CE-ICP-MS,　the　lysozyme　was　determined　within　12min　with　an　extremely　low　detection　limit　of　3.89　attomole　(3.89×10-11molL-1　for　100nL　of　sample　injection)　and　a　RSD

Keyword：

Capillary electrophoresis Enzymes Inductively coupled plasma Inductively coupled plasma mass spectrometry Mass spectrometers Proteins

Community：

[ 1 ] [Yang, MingWei]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, Fujian, China
[ 2 ] [Yang, MingWei]Department of Chemistry, Florida International University, Miami, FL 33199, United States
[ 3 ] [Wu, WeiHua]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, Fujian, China
[ 4 ] [Ruan, YaJuan]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, Fujian, China
[ 5 ] [Huang, LiMei]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, Fujian, China
[ 6 ] [Wu, Zujian]Department of Plant Protection, Fujian Agriculture and Forest University, Fuzhou 350002, Fujian, China
[ 7 ] [Cai, Yong]Department of Chemistry, Florida International University, Miami, FL 33199, United States
[ 8 ] [Fu, FengFu]Key Laboratory of Analysis and Detection for Food Safety of Ministry of Education, Fujian Provincial Key Lab of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou 350108, Fujian, China
[ 9 ] [Fu, FengFu]Department of Plant Protection, Fujian Agriculture and Forest University, Fuzhou 350002, Fujian, China

Reprint 's Address：

Email：

fengfu@fzu.edu.cn

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Related Keywords：

Ultra-sensitive quantification of lysozyme based on element chelate labeling and capillary electrophoresis inductively coupled plasma mass spectrometry
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Source ：

Analytica Chimica Acta

ISSN： 0003-2670

Year： 2014

Volume： 812

Page： 12-17

4 . 5 1 3

JCR@2014

5 . 7 0 0

JCR@2023

ESI HC Threshold：268

JCR Journal Grade：1

CAS Journal Grade：2

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 20

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 2

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