Objective:　The　strain　screened　from　nature　was　identified　and　its　laccase-producing　conditions　were　optimized　to　increase　laccase　activity.　Method:　A　strain　HYB07　with　high　yield　of　laccase　was　obtained　by　screening　with　PDA　plates　containing　guaiacol　and　Remazol　brilliant　blue　R,　respectively.　Strain　HYB07　was　identified　as　Cerrena　uni-color　via　18s　rDNA　sequence　analysis　and　morphology　observation.　Based　on　high　nitrogen　and　low　carbon　medium　(LM3),　the　carbon　sources,　nitrogen　sources,　inducer　and　initial　pH　were　optimized.　Furthermore,　the　fermentation　conditions　such　as　inoculum　volume,　temperature,　shaking　speed　and　liquid　volume　in　flask　were　explored.　The　optimal　laccase-producing　conditions　were　achieved:　glucose　2%,　Ammonium　tartrate　1.5%,　ABTS　0.05　mmol/L,　initial　pH5.0,　inoculum　volume　8%,　temperature　25°C,　shaking　speed　200　r/min,　liquid　volume　in　flask　60　mL/250　mL,　fermentation　period　6　d.　Conclusion:　Under　the　optimal　conditions,　the　maximum　laccase　activity　was　79733　U/L,　41.5%　higher　than　fermented　with　LM3　medium.　Moreover,　laccase　production　was　significantly　affected　by　Cu2+,　and　laccase　activity　was　decreased　by　87.64%　absence　of　Cu2+.