The　bonding　forms　between　β-sitosterol　and　γ-oryzanol　were　studied　by　Fourier　transform　infrared　spectrometer　(FTIS).　The　stretching　vibration　of　hydroxyl　group　of　β-sitosterol　was　shifted　obviously　to　the　lower　wave　number　as　well　as　the　carbonyl　group　of　γ-oryzanol,　which　indicated　the　hydrogen　bonds　in　β-sitosterol　and　γ-oryzanol.　The　most　powerful　hydrogen　bonds　appeared　when　the　ratio　of　β-sitosterol　and　γ-oryzanol　was　2:3,　and　the　greatest　hardness　was　also　observed　by　texture　analyzer　and　penetration　test.　The　β-sitosterol　and　γ-oryzanol　in　the　oil　phase　presented　a　new　kind　of　self-assembly　crystal　structure　with　four　petal　shape,　which　was　observed　by　polarized　light　microscopy.