The　nitrile　spherical　cellulose　adsorbent　(SCAN)　was　prepared　by　sulfomethylating　the　prepared　copolymer　of　grafting　acrylonitrile　and　acrylamide　onto　the　spherical　cellulose　beads,　doing　so　is　in　order　to　provide　reference　data　for　getting　higher　application　value　of　cellulose.　The　spherical　cellulose　beads　were　prepared　by　NMMO　(N-methylmorpholine-N-oxide)　process　and　sequential　cooling　technique.　The　sulfomethylation　process　was　optimized　through　the　orthogonal　experiments　and　single-factor　experiments.　The　results　indicate　that　the　adsorbent　prepared　has　a　specific　surface　area　of　207.9　m2　·　g-1,　and　sulfonation　degree　of　56.32%　can　be　obtained　under　following　optimal　operating　conditions:　the　solution　pH=12,　the　mass　ratio　of　HCHO,　NaHSO3　and　copolymer　is　4:6:10,　the　reaction　temperature　is　50°C　and　the　reaction　time　is　5　h.　In　addition,　the　effect　of　sulfonation　degree　on　the　adsorption　capacity　of　SCAN　was　also　studied　by　using　berberine　hydrochloride　as　adsorbate.　The　results　indicate　that　the　increase　of　sulfonation　degree　is　beneficial　to　the　adsorption　capacity,　the　maximum　adsorption　capacity　of　SCAN　could　reach　16.64　mg　·　g-1.