A　relatively　selective,　chemically　and　physically　robust　SPME　fiber　was　developed　in　a　simple　way　with　testosterone-imprinted　polymer,　and　then　directly　coupled　with　gas　chromatography-mass　spectrometry　(GC-MS)　for　selective　extraction　and　analysis　of　anabolic　steroids.　The　factors　influencing　polymerization　(i.e.,　cross-linker,　polymerization　solvent,　polymerization　time)　were　optimized　in　detail　and　the　polymer　was　characterized　by　scanning　electron　microscope,　infrared　spectrometer　and　thermogravimetric　analyzer.　Furthermore,　the　extraction　performance　of　the　MIP-coated　SPME　fibers　such　as　extraction　ability　and　selectivity　was　evaluated.　Moreover,　the　interaction　mode　between　target　analytes　and　fiber　coating　was　deducted.　Finally,　the　method　for　extraction　and　determination　of　androsterone,　stanolone,　androstenedione　and　methyltestosterone　by　the　homemade　MIP-coated　SPME　fibers　with　GC-MS　was　obtained.　It　was　applied　to　the　simultaneous　analysis　of　four　anabolic　steroids　in　the　spiked　human　urine　with　the　satisfactory　recoveries.　©　2010　Elsevier　B.V.