The　adsorption　and　aggregation　of　2-hydroxyl-propanediyl-α,ω-bis(dimethyldodecyl　ammonium　bromide)　(abbreviated　as　12-3(OH)-12)　in　aqueous　solution　have　been　investigated　by　equilibrium　and　dynamic　surface　tension,　surface　dilational　rheology,　conductivity,　fluorescence　probing,　viscosity,　zeta　potential　and　dynamic　light　scattering　measurements.　The　results　measured　by　dynamic　surface　tension,　molar　and　differential　conductivities　strongly　suggested　the　formation　of　dimolecular　moieties　in　dilute　systems　prior　to　their　adsorption　and　aggregation,　which　was　attributed　to　intermolecular　hydrogen-bonding　between　the　hydroxyl　substituted　spacers.　This　increased　the　hydrophobicity　of　the　molecular　moieties　and　greatly　promoted　their　adsorption　at　the　air/water　interface　and　association　in　solution.　The　intermolecular　hydrogen-bonding　between　the　molecules　adsorbed　at　the　air/water　interface　made　their　arrangement　tighter　and　thus　produced　lower　γcmc　compared　with　unsubstituted　12-3-12.　Moreover,　the　effect　of　intermolecular　hydrogen　bonding　promotes　growth　of　12-3(OH)-12　micelles　and　leads　to　dissociation　of　the　counterions　on　the　aggregate　surfaces.　©　2010　Elsevier　Inc.