Separation and determination of l-tyrosine and its metabolites by capillary zone electrophoresis with a wall-jet amperometric detection - Details

author：

Huang, Ying (Huang, Ying.) ^[1] | Jiang, Xiuyan (Jiang, Xiuyan.) ^[2] | Wang, Wei (Wang, Wei.) ^[3] | Duan, Jianping (Duan, Jianping.) ^[4] | Chen, Guonan (Chen, Guonan.) ^[5]

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Abstract：

A　method　of　capillary　electrophoresis　with　wall-jet　amperometric　detection　(AD)　has　been　developed　for　separation　and　determination　of　l-tyrosine　(Tyr)　and　its　metabolites,　such　as　Tyramine　(TA),　p-hydroxyphenylpyruvic　(pHPP),　homogentisic　acid　(HGA)　and　some　dipeptides　containing　Tyr,　such　as　Tyr-Gly-Gly　(YGG),　Tyr-Arg　(YR)　and　Tyr-d-Arg　(Y-d-R).　A　carbon　disk　electrode　was　used　as　the　working　electrode　and　the　optimal　detection　potential　was　1.00　V　(versus　Ag/AgCl).　At　18　kV　of　applied　voltage,　the　seven　compounds　were　completely　separated　within　20　min　in　110　×　10-3　mol/L　Na2HPO4-NaH2PO4　buffer　(pH　7.10)　containing　3　×　10-3　mol/L　β-cyclodextrin　(β-CD).　Good　linear　relationship　was　obtained　for　all　analytes　and　the　detection　limits　of　seven　analytes　were　in　the　range　of　0.95-4.25　ng/mL.　The　proposed　method　has　been　applied　to　examine　the　metabolic　process　of　l-tyrosine　in　rabbit＇s　urine.　©　2006　Elsevier　B.V.　All　rights　reserved.

Keyword：

Drug products Electric potential Electrophoresis Metabolites Pharmacokinetics Separation

Community：

[ 1 ] [Huang, Ying]The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 2 ] [Huang, Ying]College of Chemistry and Materials Science, Fujian Normal University, Fuzhou, Fujian 350007, China
[ 3 ] [Jiang, Xiuyan]The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 4 ] [Wang, Wei]The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 5 ] [Duan, Jianping]The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China
[ 6 ] [Chen, Guonan]The MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350002, China

Reprint 's Address：

Email：

gnchen@fzu.edu.cn

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Source ：

Talanta

ISSN： 0039-9140

Year： 2006

Issue： 5

Volume： 70

Page： 1157-1163

2 . 8 1

JCR@2006

5 . 6 0 0

JCR@2023

JCR Journal Grade：1

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SCOPUS Cited Count：

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 2

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