The　ability　of　diethylstilbestrol　to　enhance　the　chemiluminescence　reaction　of　luminol-H2O2,　catalyzed　by　tetrasulfonated　cobalt　phthalocyanine　in　an　alkaline　medium,　has　been　exploited　to　develop　a　new　flow　injection　chemiluminescence　method　for　the　determination　of　diethylstilbestrol.　Under　the　optimum　conditions,　the　linearity　of　the　calibration　graph　for　the　determination　of　diethylstilbestrol　was　in　the　range　from　1.00×10-7　to　4.00×10-6mol/l.　The　assay　was　sensitive　(the　detection　limit　was　6.42×10-8mol/l,　S/N=3),　reproducible　(the　relative　standard　deviation　was　2.6%,　n=11),　and　accurate　(recovery　up　to　92%).　The　method　has　been　successfully　applied　to　the　determination　of　diethylstilbestrol　in　the　pharmaceutical　formulations.　Dienestrol　and　hexestrol　were　specifically　alkylphenols,　which　are　similar　to　diethylstilbestrol,　could　be　detected　by　this　method.　Furthermore,　the　enhanced　mechanism　of　estrogen-like　compounds　was　also　discussed　in　this　paper.　©　2003　Elsevier　B.V.　All　rights　reserved.