High　performance　ion　exchange　chromatography　(HPLC)　coupled　with　laser　light　scattering　instrument　was　employed　for　analyzing　different　virulent　Ralstonia　solanacearum　strains,　and　a　new　method　for　rapidly　detecting　the　pathogenicity　of　R.　solanacearum　was　established.　The　pure　culture　of　R.　solanacearum　was　successfully　separated　into　three　characteristic　fractions　of　different　virulences　by　HPLC.　The　virulence　of　peak　3　fraction　was　the　strongest　and　that　of　peak　1　fraction　the　weakest.　Ten　strains　were　analyzed　by　HPLC　and　applied　to　infect　tomato　tissue　culture　plantlets　using　leaf-cutting　method　to　further　determine　their　virulence.　The　results　showed　that　strong　virulent　strains　formed　only　one　characteristic　peak　at　the　retention　time　of　peak　3,　which　could　make　100%　of　tissue　culture　plantlets　die　within　nine　days.　If　the　strains　were　separated　into　three　characteristic　peaks,　the　area　ratio　of　peak　3　was　increasing　with　the　virulence　of　R.　solanacearum.　Furthermore,　twenty-five　R.　solanacearum　strains　with　different　virulent　activities　were　analyzed　by　HPLC　to　validate　the　feasibility　of　this　method.　The　correlation　between　infection　mortality　of　tomato　tissue　culture　plantlets　(x)　and　peak　3　area　ratio　(y)　was　linear,　with　the　regression　equation　of　y　=　0.9581x　+　5.4984,　and　correlation　coefficient　r　=　0.986.　The　relationships　among　chromatographic　behaviors,　virulence　and　EPSI　contents　of　cell　surface　were　further　investigated　and　a　positive　correlation　was　observed　among　them.