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Abstract：
Objective To construct tobramycin overproducing strain by blocking the biosynthesis of carbamoyltobramycin and apramycin in Streptomyces tenebrarius. Methods The upstream and downstream of aprJ, a gene specific for the biosynthesis of apramycin, were cloned and used as exchange arms for homologous recombinant. The recombinant plasmid pXJ401 was conjugated into S. tenebrarius Tt49 to obtain engineering bacteria TX302. And then, tobZ coding carbamoyltransferase was disrupted to obtain engineering bacteria TX308. The change of secondary metabolites of TX302 and TX308 are detected by MS analysis. Results TX302 mainly produces carbamoyltobramycin, and no apramycin was detected in its secondary metabolites. However, TX308 produced tobramycin instead of carbamoyltobramycin. Conclusion The disruption of the phosphosugar mutase gene aprJ will block the biosynthesis of apramycin. TX308 overproduces tobramycin, and it can be used for industrial fermentative production of tobramycin.
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Chinese Journal of Antibiotics
ISSN： 1001-8689
CN： 51-1126/R
Year： 2014
Volume： 12
Page： 891-895
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ESI Highly Cited Papers on the List： 0 Unfold All
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30 Days PV： 0
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