In　this　paper,　a　novel　electrochemiluminescent　(ECL)　detection　approach　was　developed　for　highly　sensitive　detection　of　EGL　inhibitors　based　on　the　ECL　inhibition　of　Ru(bpy)32+　/2-(Dibutylamino)ethanol　(DBAE)　system.　A　microfluidic　ECL　detection　cell　was　fabricated　to　couple　with　the　capillary　electrophoresis　system,　the　electrochemical　system　and　the　post-column　injection　system.　Both　Ru(bpy)32+　and　DBAE　solutions　were　injected　directly　to　the　working　electrode　surface　by　a　micro-infusion　system　to　obtain　a　high　and　stable　ECL　signal.　The　performance　of　this　setup　was　demonstrated　by　the　analysis　of　two　typical　ECL　inhibitors,　dopamine　and　epinephrine.　Under　the　optimal　conditions,　the　limit　of　detection　(LOD)　for　dopamine　and　epinephrine　was　50　nM　and　5　nM　respectively.　The　proposed　method　was　also　successfully　used　for　the　trace　analysis　of　dopamine　and　epinephrine　in　human　serum　samples.