Objective　To　construction　the　conjugal　transfer　system　of　M.　purpurea,　and　optimize　it.　Methods　Two　exchange　arms,　which　were　respectively　located　upstream　and　downstream　of　C-methyltransferase　gene　gntK,　were　amplified　by　PCR　from　M.　purpurea　G1008　genomic　DNA.　Erythromycin　resistance　gene　(ermE,　used　as　screening　mark)　were　then　inserted　into　the　arms,　and　the　recombinant　plasmid　pFD306,　containing　the　arms　and　ermE,　for　homologous　recombination　were　constructed　from　thermal　sensitive　plasmid　pKC1139.　The　plasmid　pFD306　was　then　transformed　into　M.　purpurea　G1008　by　conjugation.　Results　One　positive　transformant　was　selected　from　the　plate　with　erythromycin,　which　was　named　GK1008.　After　PCR　identification　and　sequencing,　it　was　confirmed　that　pFD306　was　integrated　into　the　chromosome　of　G1008.　The　resistant　ability　of　the　mutant　strain　to　erythromycin　and　apramycin　were　all　more　than　500μg/mL.　Conclusion　Both　the　construction　of　the　conjugal　transfer　system　for　M.　purpurea　and　the　optimization　of　the　system　reached　the　expected　purpose,　which　provide　a　reference　for　genetic　manipulation　of　the　other　Micromonospora.