In　this　paper,　zinc　acetate,　manganese　acetate　and　thiacetamide　are　used　as　raw　materials　to　successfully　synthesize　monodispersed　ZnS:Mn2+　microspheres　by　using　hydrothermal　method　and　taking　P123　surfactant　as　a　template.　The　products　were　characterized　by　XRD,　STEM,　FT-IR　and　N2　adsorption-desorption.　And　the　results　show　that　the　diameter　of　this　microsphere　is　1.0　μm　or　so,　which　is　larger　than　that　of　ZnS　microsphere　without　Mn2+　doping,　and　it　has　monodispersion,　smooth　surface　and　uniform　size.　The　doping　of　Mn2+　does　not　obviously　change　the　structure　of　monodispersed　ZnS　microsphere.　The　photoluminescence　peak　lies　in　a　wide　band　ranging　from　450　to　650　nm,　and　the　microspheres　emit　orange　light;　with　the　increase　of　Mn2+　concentration,　fluorescence　intensity　of　ZnS:Mn2+　microsphere　changes,　and　when　the　mole　ratio　of　Mn　2+:Zn2+　is　0.3:1,　the　fluorescence　intensity　is　the　strongest.