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Abstract:
为进一步提高解淀粉芽胞杆菌α-淀粉酶(Bacillus amyloliquefaciensα-amylase,BAA)发酵生产与应用性能,对BAA编码基因实施了体外随机突变,建立了含有2×10~4个转化子的BAA突变库。BAA编码基因的突变频率为56%,基因突变效率为2.8/kb DNA,错义突变26.8%;以平板筛选法和摇瓶发酵法对BAA突变库进行筛选,得到6个酶活力显著提高的突变体,其中突变体BAA28的酶活力提高最多,提高达37%;进一步分析突变体BAA28序列,发现其4个氨基酸残基发生突变,分别是:T341P、P348L、T356P和P362L,其中T341P和T356P位于无规卷曲结...
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食品与发酵工业
Year: 2017
Issue: 10
Volume: 43
Page: 24-29
Cited Count:
SCOPUS Cited Count:
ESI Highly Cited Papers on the List: 0 Unfold All
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Chinese Cited Count:
30 Days PV: 2
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