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author:

吴吟妮 (吴吟妮.) [1] | 江贤章 (江贤章.) [2] | 潘剑茹 (潘剑茹.) [3] | 黄建忠 (黄建忠.) [4]

Abstract:

<正> 扩展青霉(Penicillium expansum)所产生的脂肪酶经硫酸氨沉淀、Sephacryl 200柱层析后,再经嗜硫色谱(Thiophilic Chromatography)柱层析,被纯化了174倍。纯化后的脂肪酶达到了SDS-PAGE电泳纯、PGAE电泳纯及毛细管反相色谱(CLC)纯。脂肪酶N-端氨基酸序列测定的结果是:A T A D A A A FP D L H R A A K L S S A。

Keyword:

分离纯化 氨基酸序列 电泳纯

Community:

  • [ 1 ] 福州大学生物工程研究所
  • [ 2 ] 福建师范大学生物工程学院

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Source :

Year: 2004

Language: Chinese

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ESI Highly Cited Papers on the List: 0 Unfold All

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Chinese Cited Count:

30 Days PV: 0

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