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author:

李仁宽 (李仁宽.) [1] | 赖庆安 (赖庆安.) [2] | 陈菁 (陈菁.) [3] | 刘树滔 (刘树滔.) [4]

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Abstract:

尝试利用毕赤酵母(pichia pastoris)表达体系大量生产制备猪胃蛋白酶,用化学的方法合成猪胃蛋白酶原A基因,并构建表达载体pGAPZα-pepsinogen;利用化学转化技术,将该基因整合到巴斯德毕赤酵母x-33菌株的染色体上.经过发酵,三角摇瓶培养条件下获得的猪胃蛋白酶原A总酶活达5 250U/L.发酵液上清经过DEAE离子交换色谱和Sephacryl S-200分子筛色谱分离,活化酶原后测定其比活达到52 800 U/g.

Keyword:

毕赤酵母 猪胃蛋白酶原 纯化 表达

Community:

  • [ 1 ] 福州大学生物工程研究所,福建福州350002

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Source :

福州大学学报:自然科学版

ISSN: 1000-2243

CN: 35-1337/N

Year: 2007

Issue: 5

Volume: 35

Page: 775-779

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count: -1

30 Days PV: 2

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