A　novel　aptamer@AuNPs@UiO-66-NH2　electrospun　nanofibrous　coating　fiber　for　specific　recognition　of　microcystin-LR　(MC-LR)　was　proposed　by　using　electrospinning,　metal-organic　frameworks　(MOF)　seed　growth　and　AuNPs　bridging　aptamer　strategies.　Characterization　of　morphology,　structure　and　stability　of　the　obtained　affinity　nanofibrous　coating　fiber　were　investigated.　High　loading　of　MOFs　and　aptamers　on　the　nanofibrous　fiber　were　achieved　and　successfully　applied　for　accurate　identification　of　MC-LR　by　solid-phase　microextraction　(SPME)　coupled　with　LC-MS.　Highly　specific　recognition　of　MC-LR　with　little　interference　of　analogs　was　achieved　with　extremely　low　LOD　(0.004　ng/mL),　good　precision　(CV%　＜　11.0%)　and　low　relative　error　(RE%　=　-1.5%　to　-10.0%),　which　was　rather　better　than　that　of　the　traditional　SPME　or　SPE　protocols.　Satisfactory　recoveries　of　MC-LR　were　obtained　in　the　range　of　92.0-96.8%　(n　=　3)　in　fortified　tap　water,　raw　pond　water　and　river　water　samples.　This　work　revealed　an　attractive　alternative　access　to　specific　recognition　and　super-sensitive　analysis　of　MC-LR　in　water.　(C)　2021　Elsevier　B.V.　All　rights　reserved.