In　this　work,　high-speed　micellar　electrokinetic　chromatography　with　LIF　detection　was　applied　to　study　the　antagonism　between　three　intestinal　bacteria,　Escherichia　coli　(E.　coli),　Bacillus　licheniformis　(B.　licheniformis)　and　Bacillus　subtilis　(B.　subtilis).　The　fluorescent　derivatization　for　the　bacteria　was　performed　by　labeling　the　bacteria　with　FITC.　In　a　high-speed　capillary　electrophoresis　(HSCE)　device,　the　three　bacteria　could　be　completely　separated　within　4　min　under　the　separation　mode　MEKC.　The　BGE　was　1　x　TBE　containing　30　mM　SDS　and　1.5　x　10(-5)　g/mL　polyethylene　oxide.　The　limits　of　detection　for　E.　coli,　B.　licheniformis　and　B.　subtilis　were　2.80　x　10(6)　CFU/mL,　1.60　x　10(6)　CFU/mL　and　1.90　x　10(6)　CFU/mL　respectively.　Lastly,　the　method　was　applied　to　investigate　the　antagonism　between　the　three　bacteria.　The　bacteria　were　mixed　and　cultured　for　7　days.　The　samples　were　separated　and　determined　every　day　to　study　the　interaction　between　bacteria.　The　results　showed　that　B.　licheniformis　and　B.　subtilis　could　not　inhibit　each　other,　but　they　could　effectively　inhibit　the　reproduction　of　E.　coli.　The　method　developed　in　this　work　was　quick,　sensitive　and　convenient,　and　it　had　great　potential　in　the　application　of　antagonism　study　for　bacteria.