• Complex
  • Title
  • Keyword
  • Abstract
  • Scholars
  • Journal
  • ISSN
  • Conference
成果搜索

author:

Xu, Liting (Xu, Liting.) [1] | Jiang, Maowang (Jiang, Maowang.) [2] | Peng, Ruibing (Peng, Ruibing.) [3] | Jiang, Xiamin (Jiang, Xiamin.) [4] | Wang, Shaoyun (Wang, Shaoyun.) [5] | Han, Qingxi (Han, Qingxi.) [6] | Zhang, Weiwei (Zhang, Weiwei.) [7]

Abstract:

The balance in bacterial community is very important for the maintenance of the health status in the hosts. During the occurrence of a pervasive skin ulcer disease in Sepia pharaonis, , bacterial isolate H1 and its antagonist bacterial isolate H2 were simultaneously isolated from the healthy cuttlefish, but only bacterial isolate H1 was isolated from the diseased cuttlefish. In the present study, the genetic and biochemical analysis showed that isolate H1 was identified as Vibrio alginolyticus, , and isolate H2 was identified as Pseudoalteromonas piscicida, , respectively. The antibiotic susceptibility test using CLSI M45-2015 method showed that the antibiotic resistance of V. alginolyticus H1 and P. piscicida H2 was different, and V. alginolyticus H1 showed strongly resistance to ampicillin. LD50 50 was calculated based the infection using immerse infection experiment. The result showed that the LD50 50 for 7 d of V. alginolyticus H1 was 1.58 x 106 6 CFU/mL, while the no death was observed during the infection of P. piscicida H2. Since the virulence related factors were significantly influenced by host immunity, the virulence factors of pathogen V. alginolyticus H1 was assessed under the stressors of H2O2 2 O 2 and 2,2 '-dipyridyl. '-dipyridyl. The results showed that the pathogenicity of V. alginolyticus H1 was associated with the haemolytic activity and bacterial motility. Different components of the P. piscicida H2 were collected and were tested for the antagonistic activity. It was unexpected that no antagonistic substance was detected, while V. alginolyticus H1 showed obvious phobotaxis to P. piscicida H2. It could be concluded that V. alginolyticus H1 was a pathogen of S. pharaonis, , and P. piscicida H2 was a potential antagonistic bacteria to inhibit V. alginolyticus H1 via chemotaxis instead of producing antagonistic substance.

Keyword:

Antagonism Balance of bacterial community Pseudoalteromonas piscicida Sepia pharaonis Vibrio alginolyticus

Community:

  • [ 1 ] [Xu, Liting]Ningbo Univ, Sch Marine Sci, 169 Qixingnan Rd, Ningbo 315832, Peoples R China
  • [ 2 ] [Jiang, Maowang]Ningbo Univ, Sch Marine Sci, 169 Qixingnan Rd, Ningbo 315832, Peoples R China
  • [ 3 ] [Peng, Ruibing]Ningbo Univ, Sch Marine Sci, 169 Qixingnan Rd, Ningbo 315832, Peoples R China
  • [ 4 ] [Jiang, Xiamin]Ningbo Univ, Sch Marine Sci, 169 Qixingnan Rd, Ningbo 315832, Peoples R China
  • [ 5 ] [Han, Qingxi]Ningbo Univ, Sch Marine Sci, 169 Qixingnan Rd, Ningbo 315832, Peoples R China
  • [ 6 ] [Zhang, Weiwei]Ningbo Univ, Sch Marine Sci, 169 Qixingnan Rd, Ningbo 315832, Peoples R China
  • [ 7 ] [Wang, Shaoyun]Fuzhou Univ, Coll Biol Sci & Technol, Fuzhou 350108, Peoples R China

Reprint 's Address:

  • [Zhang, Weiwei]Ningbo Univ, Sch Marine Sci, 169 Qixingnan Rd, Ningbo 315832, Peoples R China;;

Show more details

Related Keywords:

Source :

COMPARATIVE IMMUNOLOGY REPORTS

Year: 2024

Volume: 6

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 2

Online/Total:261/10021902
Address:FZU Library(No.2 Xuyuan Road, Fuzhou, Fujian, PRC Post Code:350116) Contact Us:0591-22865326
Copyright:FZU Library Technical Support:Beijing Aegean Software Co., Ltd. 闽ICP备05005463号-1