In　this　work,　we　rationally　design　a　new　type　of　squaraine　dye　SQ,　modified　with　the　dicyanovinyl　motif　that　can　specifically　detect　DQ　(Diquat)　by　virtue　of　aggregation-disaggregation　mechanism　and　electrostatic　interaction　between　DQ　and　SQ.　It　undergoes　H-aggregation　in　aqueous　solution,　resulting　in　fluorescence　quenching.　After　the　addition　of　DQ,　the　dicyanovinyl　group　on　the　probe　reacts　with　DQ　to　deagglomerate　so　that　the　fluorescence　is　enhanced,　tracing　DQ　in　the　environment　with　high　sensitivity　(LOD　=　4.9　×　10−7　M),　selectivity　and　good　stability.　In　addition,　the　actual　sample　spike　recovery　experiment　and　the　confocal　fluorescence　imaging　experiment　of　DQ　in　zebrafish　also　confirmed　the　detection　method.　At　present,　the　＇turn-on＇　type　fluorescence　probe　for　detecting　DQ　by　using　near-infrared　squaraine　dye　has　not　been　reported.　The　application　of　SQ　provides　a　new　strategy　for　the　quantification　of　DQ.　©　2021　Elsevier　Ltd