Activation of palindromes on a degradable modular grafting probe enables ultrasensitive detection of microRNAs - Details

author：

Xu, Jianguo (Xu, Jianguo.) ^[1] | Li, Zhi (Li, Zhi.) ^[2] | Li, Yumei (Li, Yumei.) ^[3] | Lu, Yusheng (Lu, Yusheng.) ^[4] | Wang, Jie (Wang, Jie.) ^[5]

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Abstract：

This　work　describes　a　single-stranded　degradable　modular　grafting　probe　for　analyzing　microRNA-21.　In　the　system,　the　exonuclease　activity　of　phi29　polymerase　restrains　the　SYBR　Green　I/ssDNA　induced　background.　The　palindrome　activation　caused　remarkable　target　fluorescence.　The　detection　limit　was　achieved　as　0.26　fM,　showing　potential　in　biochemical　analysis.　©　The　Royal　Society　of　Chemistry　2021.

Keyword：

Chemical activation Grafting (chemical) Linguistics Probes RNA

Community：

[ 1 ] [Xu, Jianguo]School of Food Science and Biological Engineering, Hefei University of Technology, Hefei; 230009, China
[ 2 ] [Li, Zhi]College of Chemistry, Fuzhou University, Fuzhou; 350002, China
[ 3 ] [Li, Yumei]College of Chemistry, Fuzhou University, Fuzhou; 350002, China
[ 4 ] [Lu, Yusheng]College of Chemistry, Fuzhou University, Fuzhou; 350002, China
[ 5 ] [Wang, Jie]College of Chemistry, Fuzhou University, Fuzhou; 350002, China
[ 6 ] [Wang, Jie]School of Pharmacy, Anhui Medical University, Hefei; 230031, China

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Source ：

Chemical Communications

ISSN： 1359-7345

Year： 2021

Issue： 48

Volume： 57

Page： 5941-5944

6 . 0 6 5

JCR@2021

4 . 3 0 0

JCR@2023

ESI HC Threshold：117

JCR Journal Grade：2

CAS Journal Grade：3

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 6

ESI Highly Cited Papers on the List： 0 Unfold All

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Chinese Cited Count：

30 Days PV： 2

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