Surface-enhanced　Raman　scattering　(SERS)　biosensing　has　attracted　great　attention　due　to　its　multiplexed　capability,　high　sensitivity,　and　rapid　readout.　In　this　study,　plasmonic　Ag　nanocube　(AgNC)　enhanced　SERS　biosensing　platform　was　proposed　by　coupling　nicking　endonuclease-assisted　target　recycling　signal　amplification　with　heated　electrode　for　sensitive　detection　of　DNA　species　related　to　oral　cancer.　SERS　tags　were　manufactured　by　signal　DNA　(sDNA)　conjugating　onto　the　surfaces　of　AgNCs,　and　4-mercaptobenzoic　acid　(4-MBA)　assembled　on　the　surfaces　of　AgNC/sDNA　as　a　Raman　reporter.　The　sharp　feature　of　edges　and　corners　of　AgNCs　with　high　electromagnetic　field　provided　SER　hot　spots　with　high　quality,　and　stronger　SERS　signal　could　be　achieved.　In　addition,　the　activity　of　nicking　endonuclease　Nt.BstNBI　could　be　greatly　enhanced　by　elevating　the　temperature　of　the　surface　of　the　heated　Au　electrode　(HAuE)　during　Nt.BstNBI-assisted　target　recycling　amplification　process,　resulting　in　dramatic　amplification　of　target　DNA　detection.　All　of　these　contributed　to　the　high　sensitivity　of　the　developed　SERS　biosensor.　A　limit　of　detection　(LOD)　of　3.1　fM　(S/N　=　3)　could　be　obtained　with　an　electrode　temperature　of　45　°C.　This　proposed　SERS　biosensor　was　anticipated　to　act　as　a　promising　biosensing　platform　for　early　clinical　diagnosis　of　oral　cancer.　©　2021　Elsevier　B.V.