The　assemblies　of　plasmonic　nanoparticles　(NPs)　are　the　universal　methods　for　enhancing　their　surface-enhanced　Raman　scattering　(SERS)　activities.　However,　the　present　methods　suffer　from　the　problems　of　poor　reproducibility,　complicated　fabrication,　or　the　adsorption　of　ligands　on　the　surface,　which　limit　their　practical　applications.　In　this　work,　by　using　a　facile　freeze-thaw　method,　we　are　able　to　fabricate　the　assemblies　of　Ag　NPs　with　highly　reproducible　SERS　activity　without　the　use　of　ligands.　Moreover,　the　Ag　NPs　can　be　well　kept　in　a　frozen　state　for　a　long　time　with　few　influences　on　the　reproducibility　(relative　standard　deviation,　RSD　ca.　7%),　while　those　kept　in　colloid　(4　°C)　suffer　from　gradual　surface　oxidation　and　aggregation.　Such　a　simple　freeze-thaw　method　does　not　require　the　introduction　of　any　ligands　(or　linkers)　with　long-term　stability　and　reproducibility,　implying　its　wide　applications　in　practical　SERS　sensing.　©