Recently　emerging　perovskite　quantum　dots　(PQDs)　with　extremely　high　(near　100%)　fluorescence　quantum　yield　(FQY)　may　have　attractive　applications　in　sensing　and　bioimaging.　However,　practical　applications　of　PQDs　as　signal　probes　in　aqueous　media,　especially　in　long-time　bioimaging,　have　been　greatly　limited　by　the　well-known　poor　stability　of　PQDs　in　water.　In　this　work,　a　single　intact　CsPbBr3　PQD　nanocrystal　was　well　encapsulated　with　a　SiO2　shell　using　a　simple　and　highly　successful　coating　strategy.　In　the　synthesis,　polyvinyl　pyrrolidone　(PVP)　instead　of　classic　oleic　acid　(OA)　and　oleylamine　(OAm)　was　used　as　the　ligand　and　surfactant　to　increase　the　affinity　between　PQDs　and　SiO2,　significantly　increasing　the　coating　efficiency　of　PQD　single　nanocrystals.　Meanwhile,　easily　hydrolyzed　trimethoxysilane　(TMOS)　was　adopted　as　the　silicon　source　to　shorten　the　hydrolysis　time　of　silane,　which　obviously　decreased　the　degradation　of　PQDs　during　SiO2　coating.　The　obtained　PVP-PQD@SiO2　core-shell　nanoparticles　were　uniform　in　size,　maintained　a　high　FQY　of　PQDs,　and　exhibited　excellent　air　and　ultraviolet　(UV)　stability.　Moreover,　PVP-PQD@SiO2　core-shell　nanoparticles　could　be　easily　modified　with　trimethoxyoctadecylsilane　(C18)　and　lecithin,　in　turn,　to　assemble　a　lipid　layer　on　their　surfaces,　yielding　very　well　water-dispersed,　long-term　water-stable,　highly　fluorescent,　and　low-cytotoxic　PVP-PQD@SiO2@C18-PC　nanoprobes.　It　was　demonstrated　that　these　good　properties　of　the　obtained　PVP-PQD@SiO2@C18-PC　nanoprobes　enabled　their　successful　application　in　cell　imaging.　©　2021　American　Chemical　Society.　All　rights　reserved.