The　emerging　technique　of　carbonization　of　polyimide　(PI)　by　direct　laser　writing　receives　great　attention　for　its　flexibility,　versatility,　and　ease-of-patterning　capability　in　creating　a　variety　of　functional　laser-induced　graphene　(LIG)　sensors　and　devices.　LIG　prepared　by　CO2　laser　irradiating　of　the　PI　film　is　characterized　by　scanning　electron　microscopy　(SEM),　X-ray　diffraction　(XRD),　transmission　electron　microscope　(TEM),　specific　surface　area　analyzer,　synchronous　thermal　analysis,　and　Raman　spectroscopy　with　the　focus　on　investigating　the　effects　of　laser　parameters　(e.g.,　power,　scanning　speed)　on　the　microstructure,　thickness,　and　sheet　resistance　of　LIG.　Both　TEM　and　XRD　indicate　that　LIG　is　composed　of　many　graphene　layers　with　a　layer　spacing　of　0.34　nm.　The　specific　surface　area　of　LIG　decreases　with　the　increase　of　laser　power.　The　ratio　of　the　thickness　of　LIG　over　the　depth　of　the　carbonized　PI　film　as　the　expansion　ratio　characterizes　the　expansibility　of　LIG.　The　influence　of　image　resolution　and　off-focus　value　on　the　sheet　resistance　of　LIG　is　explained　by　the　superposition　mechanism　of　laser　scanning　spots.　©　2021,　Science　China　Press　and　Springer-Verlag　GmbH　Germany,　part　of　Springer　Nature.