This　work　presented　a　point-of-care　(POC)　photo-electrochemical　(PEC)　biosensing　for　the　detection　of　human　papillomavirus-16　(HPV-16)　on　a　portable　electrochemical　detection　system　by　using　CRISPR-Cas12a　trans-cleaving　the　G-quadruplex　for　the　biorecognition/amplification　and　a　hollow　In2O3-In2S3-modified　screen-printed　electrode　(In2O3-In2S3/SPE)　as　the　photoactive　material.　G-quadruplexes　were　capable　of　biocatalytic　precipitation　(H2O2-mediated　4-chloro-1-naphthol　oxidation)　on　the　In2O3-In2S3/SPE　surface,　resulting　in　a　weakened　photocurrent,　but　suffered　from　transcleavage　when　the　CRISPR-Cas12a　system　specifically　recognized　the　analyte.　The　photocurrent　results　could　be　directly　observed　with　the　card-sized　electrochemical　device　via　a　smartphone,　which　displayed　a　high-value　photocurrent　for　these　positive　samples,　while　a　low-value　photocurrent　for　the　target-free　samples.　Such　a　system　exhibited　satisfying　photocurrent　responses　toward　HPV-16　within　a　wide　working　range　from　5.0　to　5000　pM　and　allowed　for　detection　of　HPV-16　at　a　concentration　as　low　as　1.2　pM.　The　proposed　assay　provided　a　smartphone　signal　readout　to　enable　the　rapid　screening　PEC　determination　of　HPV-16　concentration　without　sophisticated　instruments,　thus　meeting　the　requirements　of　remote　areas　and　resource-limited　settings.　We　envision　that　combining　an　efficient　biometric　PEC　sensing　platform　with　a　wireless　card-sized　electrochemical　device　will　enable　high-throughput　POC　diagnostic　analysis.