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author:

Huang, Shuoying (Huang, Shuoying.) [1] | Lai, Wenqiang (Lai, Wenqiang.) [2] | Liu, Bingqian (Liu, Bingqian.) [3] | Xu, Mingdi (Xu, Mingdi.) [4] | Zhuang, Junyang (Zhuang, Junyang.) [5] | Tang, Dianping (Tang, Dianping.) [6] (Scholars:唐点平) | Lin, Youxiu (Lin, Youxiu.) [7]

Indexed by:

EI Scopus SCIE

Abstract:

In this work, a split-type dual-mode (colorimetric/photothermal) immunoassay method was designed for point-of-care testing (POCT) detection of mycotoxins (aflatoxin B-1, AFB(1) as the model analyte) in foodstuffs based on Pt supported on nitrogen-doped carbon amorphous (Pt-CN). The as-synthesized Pt-CN exhibits excellent peroxidase-mimicking activity, which can catalyze the oxidization of 3,3 & PRIME;,5,5 & PRIME;-tetramethylbenzidine (TMB) into TMBox with sensitive colorimetric readout in the presence of hydrogen peroxide (H2O2). Moreover, the TMBox also serves as a near-infrared (NIR) photothermal agent to convert the colorimetric readout into heat under the irradiation of an 808 nm laser. A competitive-type immunoreaction is carried out between AFB(1) and glucose oxidase (GOx)-labeled AFB(1)-bovine serum albumin (AFB(1)-BSA-GOx) conjugates. With the formation of immune complexes, the entrained GOx catalyzes the hydrolysis of glucose to generate H2O2, which further involves the Pt-CN catalyzed production of TMBox to increase colorimetric/photothermal readouts. Depending on the degree of TMB oxidation, the dual-mode immunoassay provides a linear range of 1.0 pg/mL to 10 ng/mL, with a limit of detection (LOD) of 0.22 pg/mL for the colorimetric assay and 0.76 pg/mL for the photothermal assay. Moreover, the developed method is successfully used to detect AFB1 in peanuts with acceptable accuracy compared with commercially enzyme-linked immunosorbent assay (ELISA) kits. Significantly, the photothermal readout in this method is recorded on a mobile phone device without any expensive instruments, providing an affordable and convenient tool for food safety testing.

Keyword:

AflatoxinB(1) Dual readout Photothermal and colorimetric Pt supported on nitrogen-doped carbon

Community:

  • [ 1 ] [Huang, Shuoying]Minnan Normal Univ, Coll Chem, Key Lab Modern Analyt Sci & Separat Technol Fujian, Key Lab Pollut Monitoring & Control Fujian Prov, Zhangzhou 363000, Peoples R China
  • [ 2 ] [Lai, Wenqiang]Minnan Normal Univ, Coll Chem, Key Lab Modern Analyt Sci & Separat Technol Fujian, Key Lab Pollut Monitoring & Control Fujian Prov, Zhangzhou 363000, Peoples R China
  • [ 3 ] [Lin, Youxiu]Minnan Normal Univ, Coll Chem, Key Lab Modern Analyt Sci & Separat Technol Fujian, Key Lab Pollut Monitoring & Control Fujian Prov, Zhangzhou 363000, Peoples R China
  • [ 4 ] [Liu, Bingqian]Guizhou Univ, Coll Pharm, Guizhou Engn Lab Synthet Drugs, Minist Educ Guizhou Prov, Guiyang 550025, Peoples R China
  • [ 5 ] [Xu, Mingdi]Fujian Univ Technol, Coll Ecol Environm & Urban Construct, Fuzhou 350118, Peoples R China
  • [ 6 ] [Zhuang, Junyang]Fujian Med Univ, Sch Pharm, Higher Educ Key Lab Nano Biomed Technol Fujian Pro, Fuzhou 350108, Peoples R China
  • [ 7 ] [Tang, Dianping]Fuzhou Univ, Inst Nanomed & Nanobiosensing, Dept Chem, Key Lab Anal & Detect Food Safety MOE & Fujian Pro, Fuzhou 350108, Peoples R China

Reprint 's Address:

  • [Lin, Youxiu]Minnan Normal Univ, Coll Chem, Key Lab Modern Analyt Sci & Separat Technol Fujian, Key Lab Pollut Monitoring & Control Fujian Prov, Zhangzhou 363000, Peoples R China;;

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Source :

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY

ISSN: 1386-1425

Year: 2023

Volume: 284

4 . 3

JCR@2023

4 . 3 0 0

JCR@2023

ESI Discipline: CHEMISTRY;

ESI HC Threshold:39

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count: 19

SCOPUS Cited Count:

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 4

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