DNAzyme-based　signal　amplification　circuits　promote　the　advances　in　low-abundant　miRNA　imaging　in　living　cells.　However,　due　to　the　insufficient　cofactor　in　living　cells　and　unsustainable　target　utilization,　self-powered　and　self-feedback　DNAzyme　amplification　circuits　have　rarely　been　achieved.　Here,　a　MnO2　nanosheet-mediated　self-powered　and　self-feedback　entropy-driven　catalyst　(EDC)-DNAzyme　nanoprobe　(MnPFEDz)　was　demonstrated　for　sensitive　imaging　of　intracellular　microRNA　(miRNA).　In　this　strategy,　MnPFEDz　was　formed　by　adsorbing　EDC　modules　and　substrate　probes　on　MnO2　nanosheets.　The　MnO2　nanosheets　acted　not　only　as　glutathione　(GSH)-responsive　nanocarriers　for　efficient　delivery　of　DNA　probes　but　also　as　a　DNAzyme　cofactor　supplier　to　power　the　DNAzyme　biocatalysis　and　promote　signal　transduction　in　a　feedback　way.　When　entering　the　cells,　GSH　could　decompose　MnO2　nanosheets　to　generate　numerous　Mn2+　ion　cofactors,　leading　to　the　release　of　DNA　probes.　Subsequently,　the　target　miRNA　initiated　EDC　cycles　to　generate　amplified　fluorescence　signals　and　exposed　the　complete　DNAzyme.　Meanwhile,　each　of　the　exposed　DNAzyme　then　cleaved　the　substrate　probes　with　the　help　of　Mn2+　ion　cofactors　and　released　a　new　trigger　analogue　for　the　next　round　of　EDC　cycles,　initiating　additional　fluorescence　signals　in　a　feedback　way.　As　a　multiple　signal　amplification　strategy,　the　MnPFEDz　nanoprobe　facilitated　the　effective　detection　of　intracellular　molecules　with　enhanced　sensitivity　and　provided　a　versatile　strategy　for　the　construction　of　self-powered　and　self-feedback　DNA　circuits　in　living　cells.　©　2022　American　Chemical　Society.