In　this　work,　a　facile　separation　of　self-floating　Ag3VO4/BiVO(4　)hydrogel　(ABVH)　photocatalyst　was　constructed　via　the　freeze-thaw　method　for　the　inactivation　of　Microcystis　aeruginosa.　The　as-synthesized　photocatalyst　exhibited　excellent　photocatalytic　performance,　resulting　in　the　degradation　efficiency　of　chlorophyll　a　reaching　approximately　99.6%　within　4　h　under　visible　light.　The　cell　morphology,　intracellular　organic　matter　(IOM),　extracellular　organic　matter　(EOM),　and　antioxidant　system　were　severely　disrupted　during　the　photocatalytic　process.　The　inactivation　mechanism　could　be　ascribed　to　the　center　dot　OH　and　h(+)　generated　by　hydrogel　photocatalyst　under　visible　light　irradiation.　Furthermore,　five　repetitive　experiments　and　cyclic　compressive　stress-strain　tests　demonstrated　that　the　as-prepared　hydrogel　possessed　excellent　reusability　and　mechanical　strength　properties.　Besides,　the　hydrogel　photocatalyst　showed　excellent　potential　for　the　removal　of　M.　aeruginosa　in　the　presence　of　natural　organic　matter　(NOM).　Overall,　the　synthesized　self-floating　hydrogel　photocatalyst　may　offer　a　novel　insight　into　the　control　of　harmful　cyanobacterial　blooms.