Traditional　separation　materials　based　on　Ti3C2Tx　modification　are　susceptible　to　contamination　by　oily　sub-stances　and　have　single　performance,　which　limits　their　applications　in　treatment　of　actual　oily　wastewater.　Here,　to　improve　the　hydrophilicity　and　stability　of　Ti3C2Tx,　EHL-functionalized　Ti3C2Tx　(EHL-Ti3C2Tx)　was　prepared　by　enzymatic　hydrolysis　lignin　(EHL)　forming　secondary　bonds　with　the　terminal　oxygen-containing　groups　on　the　surface　of　Ti3C2Tx.　EHL-Ti3C2Tx　was　further　coated　on　polyurethane　(PU)　sponge　to　construct　rough　structures,　enabling　the　modified　EHL-Ti3C2Tx　@PU　sponge　superhydrophilic/underwater　super-oleophobic　wettability　to　achieve　separation　efficiency　above　99.5%　for　different　oil/water　mixtures.　Especially,　the　maximum　permeation　flux　of　EHL-Ti3C2Tx　@PU　for　cyclohexane/water　mixture　separation　is　up　to　231,321　L　m-　2　h-1.　In　addition,　the　functionalized　interface　constructed　by　EHL-Ti3C2Tx　on　mixed　cellulose　ester　(MCE)　membrane　exhibits　high　permeability　to　malachite　green　and　methylene　blue　cationic　dyes　with　rejection　rates　of　99.1%　and　98.1%,　respectively.　Therefore,　two　separation　materials　obtained　by　decorating　EHL-Ti3C2Tx　on　PU　and　MCE　substrates　have　extremely　potential　in　treating　complex　wastewater.