Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA - Details

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Abstract：

Herein,　a　versatile　fluorescent　bioanalysis　platform　for　sensitive　and　specific　screening　of　target　miRNA　(miR-129-2-3p)　was　innovatively　designed　by　applying　target-induced　rolling　circle　amplification　(RCA)　for　efficient　signal　amplification.　Specifically,　miR-　129-2-3p　was　used　as　a　ligation　template　to　facilitate　its　ligation　with　padlock　probes,　followed　by　an　RCA　reaction　in　the　presence　of　phi29　DNA　polymerase.　The　dsDNA　fragments　and　products　were　stained　by　SYBR　Green　I　and　then　detected　by　fluorescence　spectrophotometry.　As　a　result,　miR-129-2-3p　concentrations　as　low　as　50　nM　could　be　detected.　Furthermore,　the　expression　of　miR-129-2-3p　in　breast　cancer　patients　was　about　twice　that　in　healthy　people.　Therefore,　the　results　indicated　that　the　RCA-based　biosensor　system　could　be　a　valuable　platform　for　miRNA　detection　in　clinical　diagnosis　and　biomedical　study.　©　2022　Ye　et　al.

Keyword：

Biosensor Fluorescent detection miR-129-2-3p RCA

Community：

[ 1 ] [Ye, Y.]Fujian Normal University, College of Life Sciences, Fujian, Fuzhou, China
[ 2 ] [Lin, Y.]Cooperation Base of Traditional Chinese Medicine-Oriented Chronic Disease Prevention and Treatment, Innovation and Transformation Center, Fujian University of Traditional Chinese Medicine, Fuzhou, China
[ 3 ] [Chi, Z.]Fujian Normal University, College of Life Sciences, Fujian, Fuzhou, China
[ 4 ] [Zhang, J.]The First Affiliated Hospital of Fujian Medical University, Department of Thyroid and Breast Surgery, Fujian, Fuzhou, China
[ 5 ] [Cai, F.]Fujian Normal University, College of Life Sciences, Fujian, Fuzhou, China
[ 6 ] [Zhu, Y.]The First Affiliated Hospital of Fujian Medical University, Department of Thyroid and Breast Surgery, Fujian, Fuzhou, China
[ 7 ] [Tang, D.]Fuzhou University, Key Laboratory for Analytical Science of Food Safety and Biology (MOE & Fujian Province), State Key Laboratory of Photocatalysis on Energy and Environment, Department of Chemistry, Fujian, Fuzhou, China
[ 8 ] [Lin, Q.]Fujian Normal University, College of Life Sciences, Fujian, Fuzhou, China

Reprint 's Address：

[Lin, Q.]Fujian Normal University, Fujian, China;;[Tang, D.]Fuzhou University, Fujian, China

Email：

qingqianglin@fjnu.edu.cn |
dianping.tang@fzu.edu.cn

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Related Keywords：

Rolling circle amplification (RCA) -based biosensor system for the fluorescent detection of miR-129-2-3p miRNA
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Source ：

PeerJ

ISSN： 2167-8359

Year： 2022

Volume： 10

2 . 7

JCR@2022

2 . 3 0 0

JCR@2023

ESI HC Threshold：117

JCR Journal Grade：2

CAS Journal Grade：3

Cited Count：

WoS CC Cited Count：

SCOPUS Cited Count： 2

ESI Highly Cited Papers on the List： 0 Unfold All

WanFang Cited Count：

Chinese Cited Count：

30 Days PV： 1

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