Herein,　a　versatile　fluorescent　bioanalysis　platform　for　sensitive　and　specific　screening　of　target　miRNA　(miR-129-2-3p)　was　innovatively　designed　by　applying　target-induced　rolling　circle　amplification　(RCA)　for　efficient　signal　amplification.　Specifically,　miR129-2-3p　was　used　as　a　ligation　template　to　facilitate　its　ligation　with　padlock　probes,　followed　by　an　RCA　reaction　in　the　presence　of　phi29　DNA　polymerase.　The　dsDNA　fragments　and　products　were　stained　by　SYBR　Green　I　and　then　detected　by　fluorescence　spectrophotometry.　As　a　result,　miR-129-2-3p　concentrations　as　low　as　50　nM　could　be　detected.　Furthermore,　the　expression　of　miR-129-2-3p　in　breast　cancer　patients　was　about　twice　that　in　healthy　people.　Therefore,　the　results　indicated　that　the　RCA-based　biosensor　system　could　be　a　valuable　platform　for　miRNA　detection　in　clinical　diagnosis　and　biomedical　study.