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author:

Zhang, Xu (Zhang, Xu.) [1] | Zhou, Zixuan (Zhou, Zixuan.) [2] | Dai, Lin (Dai, Lin.) [3] | Chao, Yulin (Chao, Yulin.) [4] | Liu, Zheng (Liu, Zheng.) [5] | Huang, Mingdong (Huang, Mingdong.) [6] (Scholars:黄明东) | Qu, Qianhui (Qu, Qianhui.) [7] | Lin, Zhonghui (Lin, Zhonghui.) [8] (Scholars:林忠辉)

Indexed by:

Scopus SCIE

Abstract:

Holliday junction (HJ) is a four-way structured DNA intermediate in homologous recombination. In bacteria, the HJ-specific binding protein RuvA and the motor protein RuvB together form the RuvAB complex to catalyze HJ branch migration. Pseudomonas aeruginosa (P. aeruginosa, Pa) is a ubiquitous opportunistic bacterial pathogen that can cause serious infection in a variety of host species, including vertebrate animals, insects and plants. Here, we describe the cryo-Electron Microscopy (cryo-EM) structure of the RuvAB-HJ intermediate complex from P. aeruginosa. The structure shows that two RuvA tetramers sandwich HJ at the junction center and disrupt base pairs at the branch points of RuvB-free HJ arms. Eight RuvB subunits are recruited by the RuvA octameric core and form two open-rings to encircle two opposite HJ arms. Each RuvB subunit individually binds a RuvA domain III. The four RuvB subunits within the ring display distinct subdomain conformations, and two of them engage the central DNA duplex at both strands with their C-terminal beta-hairpins. Together with the biochemical analyses, our structure implicates a potential mechanism of RuvB motor assembly onto HJ DNA.

Keyword:

branch migration complex assembly DNA damage repair Holliday junction homologous recombination Pseudomonas aeruginosa RuvA RuvB

Community:

  • [ 1 ] [Zhang, Xu]Fuzhou Univ, Coll Chem, Fuzhou, Peoples R China
  • [ 2 ] [Dai, Lin]Fuzhou Univ, Coll Chem, Fuzhou, Peoples R China
  • [ 3 ] [Huang, Mingdong]Fuzhou Univ, Coll Chem, Fuzhou, Peoples R China
  • [ 4 ] [Lin, Zhonghui]Fuzhou Univ, Coll Chem, Fuzhou, Peoples R China
  • [ 5 ] [Zhou, Zixuan]Fudan Univ, Shanghai Stomatol Hosp, Inst Biomed Sci, Dept Syst Biol Med, Shanghai, Peoples R China
  • [ 6 ] [Chao, Yulin]Fudan Univ, Shanghai Stomatol Hosp, Inst Biomed Sci, Dept Syst Biol Med, Shanghai, Peoples R China
  • [ 7 ] [Qu, Qianhui]Fudan Univ, Shanghai Stomatol Hosp, Inst Biomed Sci, Dept Syst Biol Med, Shanghai, Peoples R China
  • [ 8 ] [Liu, Zheng]Chinese Univ Hong Kong, Kobilka Inst Innovat Drug Discovery, Sch Med, Shenzhen, Peoples R China

Reprint 's Address:

  • [Lin, Zhonghui]Fuzhou Univ, Coll Chem, Fuzhou, Peoples R China;;[Qu, Qianhui]Fudan Univ, Shanghai Stomatol Hosp, Inst Biomed Sci, Dept Syst Biol Med, Shanghai, Peoples R China;;

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Source :

FRONTIERS IN PLANT SCIENCE

ISSN: 1664-462X

Year: 2023

Volume: 14

4 . 1

JCR@2023

4 . 1 0 0

JCR@2023

ESI Discipline: PLANT & ANIMAL SCIENCE;

ESI HC Threshold:18

JCR Journal Grade:1

CAS Journal Grade:2

Cited Count:

WoS CC Cited Count: 3

SCOPUS Cited Count: 2

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 0

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