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author:

Sun, Hao (Sun, Hao.) [1] (Scholars:孙浩) | Jiang, Qinghua (Jiang, Qinghua.) [2] | Huang, Yi (Huang, Yi.) [3] | Mo, Jin (Mo, Jin.) [4] | Xie, Wantao (Xie, Wantao.) [5] | Dong, Hui (Dong, Hui.) [6] | Jia, Yuan (Jia, Yuan.) [7]

Indexed by:

EI Scopus SCIE

Abstract:

Pandemics such as COVID-19 have exposed global inequalities in essential health care. Here, we proposed a novel analytics of nucleic acid amplification tests (NAATs) by combining paper microfluidics with deep learning and cloud computing. Real-time amplifications of synthesized SARS-CoV-2 RNA templates were performed in paper devices. Information pertained to on-chip reactions in time-series format were transmitted to cloud server on which deep learning (DL) models were preloaded for data analysis. DL models enable prediction of NAAT results using partly gathered real-time fluorescence data. Using information provided by the G-channel, accurate prediction can be made as early as 9 min, a 78% reduction from the conventional 40 min mark. Reaction dy-namics hidden in amplification curves were effectively leveraged. Positive and negative samples can be unbiasedly and automatically distinguished. Practical utility of the approach was validated by cross-platform study using clinical datasets. Predicted clinical accuracy, sensitivity and specificity were 98.6%, 97.6% and 99.1%. Not only the approach reduced the need for the use of bulky apparatus, but also provided intelligent, distributable and robotic insights for NAAT analysis. It set a novel paradigm for analyzing NAATs, and can be combined with the most cutting-edge technologies in fields of biosensor, artificial intelligence and cloud computing to facilitate fundamental and clinical research.

Keyword:

Cloud computing Deep learning NAAT Paper microfluidics Time-series forecasting

Community:

  • [ 1 ] [Sun, Hao]Fuzhou Univ, Sch Mech Engn & Automat, Fuzhou 350116, Peoples R China
  • [ 2 ] [Mo, Jin]Fuzhou Univ, Sch Mech Engn & Automat, Fuzhou 350116, Peoples R China
  • [ 3 ] [Xie, Wantao]Fuzhou Univ, Sch Mech Engn & Automat, Fuzhou 350116, Peoples R China
  • [ 4 ] [Dong, Hui]Fuzhou Univ, Sch Mech Engn & Automat, Fuzhou 350116, Peoples R China
  • [ 5 ] [Jiang, Qinghua]Harbin Inst Technol, Sch Life Sci & Technol, Harbin 150001, Peoples R China
  • [ 6 ] [Huang, Yi]Fujian Prov Hosp, Ctr Expt Res Clin Med, Fuzhou 350001, Peoples R China
  • [ 7 ] [Dong, Hui]Fujian Prov Collaborat Innovat Ctr High End Equipm, Fuzhou 350116, Peoples R China
  • [ 8 ] [Jia, Yuan]Shenzhen Technol Univ, Sino German Coll Intelligent Mfg, Shenzhen 518118, Peoples R China

Reprint 's Address:

  • [Sun, Hao]Fuzhou Univ, Sch Mech Engn & Automat, Fuzhou 350116, Peoples R China;;

Email:

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Source :

BIOMEDICAL SIGNAL PROCESSING AND CONTROL

ISSN: 1746-8094

Year: 2023

Volume: 83

4 . 9

JCR@2023

4 . 9 0 0

JCR@2023

ESI Discipline: ENGINEERING;

ESI HC Threshold:35

JCR Journal Grade:1

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count: 8

SCOPUS Cited Count: 4

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 1

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