Bacillus　subtilis　TY-1　was　isolated　from　2000　m-deep　sea　sediments　of　the　Western　Pacific　Ocean,　which　was　found　to　exhibit　strong　antagonistic　activity　against　tobacco　bacterial　wilt　caused　by　Ralstonia　solanacearum.　Here,　we　present　the　annotated　complete　genomic　sequence　of　the　strain　Bacillus　subtilis　TY-1.　The　genome　consists　of　a　4,030,869-bp　circular　chromosome　with　a　G　+　C　content　of　43.88%,　86　tRNAs,　and　30　rRNAs.　Genomic　analysis　identified　a　large　number　of　gene　clusters　involved　in　the　biosynthesis　of　antibacterial　metabolites,　including　lipopeptides(surfactin,　bacillibactin,　and　fengycin)　and　polyketides(bacillaene).　Meanwhile,　numerous　genes　encoding　carbohydrate-active　enzymes　and　secreted　proteins　were　found　in　TY-1.　These　findings　suggest　that　Bacillus　subtilis　TY-1　appears　to　be　a　potential　biocontrol　agent　against　tobacco　bacterial　wilt　in　agricultural　fields.　©　2023　Elsevier　B.V.