Helicobacter　pylori　is　closely　linked　to　many　gastric　diseases　such　as　gastric　ulcers　and　duodenal　ulcers.　Therefore,　biosensing　H.　pylori　has　attracted　wide　attention　from　both　scientists　and　clinicians.　Here,　we　proposed　an　electrochemiluminescence　(ECL)-based　platform　that　could　sensitively　detect　H.　pylori　DNA.　In　this　platform,　a　novel　target-cycling　synchronized　rolling　circle　amplification　was　used　for　signal　amplification.　Silver　nanoclusters　(Ag　NCs)　were　synthesized　on　the　circle　DNA　products,　embedding　them　with　the　ability　to　catalyze　the　electrochemical　reduction　of　K2S2O8,　in　turn　resulting　in　rapid　consumption　of　the　ECL　co-reactant　near　the　working　electrode,　and　leading　to　a　decrease　in　the　ECL　emission　intensity.　In　addition　to　its　excellent　stability　and　selectivity,　the　proposed　strategy　had　a　low　detection　limit　of　10　pM,　an　indication　that　it　can　be　beneficially　applied　to　test　biosamples.　Furthermore,　a　biosensing　chip　was　designed　to　improve　the　throughput　and　shed　new　light　on　large-scale　clinical　biosensing　applications.　©　2023　John　Wiley　&　Sons　Ltd.