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author:

Xu, Liangzhen (Xu, Liangzhen.) [1] | Hou, Shuang (Hou, Shuang.) [2] | Huang, Xiaocui (Huang, Xiaocui.) [3] | Wang, Mengyuan (Wang, Mengyuan.) [4] | Li, Cheng (Li, Cheng.) [5] | Dong, Nuo (Dong, Nuo.) [6] | Lin, Zhenyu (Lin, Zhenyu.) [7]

Indexed by:

EI

Abstract:

MicroRNA-155 (miR-155) can act as a suitable biomarker for dry eye diseases caused by Sjögren's syndrome. Since the concentration of miR-155 in the clinic samples is low, it is necessary to develop some simple but sensitive detection methods. In this study, a homogeneous electrochemiluminescence (ECL) biosensor for miR-155 detection based on catalyze hairpin assembly (CHA) and hybridization chain reaction (HCR) and magnetic assisted enrichment had been constructed. Firstly, biotin-modified hairpin H1 (bio-H1) had been connected with the streptavidin-modified magnetic beads (SAMBs) to capture target, which can be used to trigger CHA reaction to complete the first signal amplification with the assistance of hairpin H2. Then the stick end of H2 of the CHA production can trigger the HCR reaction of H3 and H4 and generate long double-stranded DNAs (dsDNAs) on the surface of SAMBs to realize the second signal amplification. Ru(phen)32+ can insert into the dsDNAs to produce SAMBs/dsDNA/Ru and act as signal tags, which can be purified by magnetic separation easily, making the background signal significantly reduced. The ECL intensity of the system had a linear relationship with logarithm of miR-155 concentration in the range of 1 fM ∼ 10 nM, with a detection limit as low as 0.63 fM. The proposed biosensor had been applied to monitor miR-155 in clinical samples with satisfied results. © 2023 Elsevier B.V.

Keyword:

Amplification Biosensors Magnetic separation Proteins RNA

Community:

  • [ 1 ] [Xu, Liangzhen]Eye Institute & Affiliated Xiamen Eye Center, School of Medicine, Xiamen University, Fujian, Xiamen, China
  • [ 2 ] [Hou, Shuang]Eye Institute & Affiliated Xiamen Eye Center, School of Medicine, Xiamen University, Fujian, Xiamen, China
  • [ 3 ] [Huang, Xiaocui]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, China
  • [ 4 ] [Wang, Mengyuan]Eye Institute & Affiliated Xiamen Eye Center, School of Medicine, Xiamen University, Fujian, Xiamen, China
  • [ 5 ] [Li, Cheng]Eye Institute & Affiliated Xiamen Eye Center, School of Medicine, Xiamen University, Fujian, Xiamen, China
  • [ 6 ] [Dong, Nuo]Eye Institute & Affiliated Xiamen Eye Center, School of Medicine, Xiamen University, Fujian, Xiamen, China
  • [ 7 ] [Dong, Nuo]Zhenjiang College, Jiangsu, Zhenjiang, China
  • [ 8 ] [Dong, Nuo]Fujian Provincial Key Laboratory of Ocular Surface and Corneal Diseases, Fujian, Xiamen, China
  • [ 9 ] [Lin, Zhenyu]Ministry of Education Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection for Food Safety, College of Chemistry, Fuzhou University, Fujian, Fuzhou, China

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Source :

Journal of Electroanalytical Chemistry

ISSN: 1572-6657

Year: 2023

Volume: 933

4 . 1

JCR@2023

4 . 1 0 0

JCR@2023

ESI HC Threshold:39

JCR Journal Grade:1

CAS Journal Grade:3

Cited Count:

WoS CC Cited Count:

SCOPUS Cited Count: 4

ESI Highly Cited Papers on the List: 0 Unfold All

WanFang Cited Count:

Chinese Cited Count:

30 Days PV: 3

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