Based　on　the　pre-column　derivatization　of　monosaccharides　with　1-phenyl-3-methyl-5-pyrazolonethe　fingerprints　of　polysaccharide　hydrolysate　monosaccharides　in　the　extract　of　Mesona　chinensis　Benth　was　established　using　reversed-phase　high　performance　liquid　chromatography　HPLC　gradient　elution/UV　detection　technologywhich　was　used　for　the　analysis　of　the　components　and　content　of　polysaccharide　hydrolyzed　monosaccharides　in　the　aqueous　extract　of　Mesona　chinensis　Benthand　eight　monosaccharide　components　including　riboserhamnoseglucuronic　acidgalacturonic　acidglucosegalactosexylose　and　arabinose　were　identified.　Similarity　analysiscluster　analysisprincipal　component　analysis　of　the　HPLC　fingerprints　of　monosaccharide　components　were　carried　outand　the　changes　in　the　contents　of　galactose　galacturonic　acid　and　glucose　monosaccharide　components　in　various　samples　were　clarified.　Regional　differences　lead　to　differences　in　cluster　analysisand　there　are　certain　differences　in　the　content　and　distribution　of　monosaccharide　components　produced　by　polysaccharide　hydrolysis　in　Mesona　chinensis　Benth　collected　from　different　origins.　The　method　can　provide　a　potential　technology　for　characterizing　monosaccharide　components　of　the　polysaccharide　in　aqueous　extract　of　Mesona　chinensis　Benth.　©　2023,　Youke　Publishing　Co.,Ltd.　All　rights　reserved.