Rhizome　of　Polygonatum　sibiricum　is　both　a　renowned　traditional　Chinese　remedy　and　a　commonly　consumed　delicacy.　Due　to　the　escalating　demand　and　excessive　overexploitation,　there　has　been　a　growing　interest　in　the　artificial　cultivation　of　this　plant　in　recent　years.　To　assess　the　therapeutic　benefits　of　artificially　cultivated　P.　sibiricum,　it　is　crucial　to　identify　and　classify　its　phytochemical　components,　which　are　the　primary　bioactive　compounds　found　in　its　rhizome.　In　this　study,　the　phytochemical　profile　of　an　artificially　cultivated　P.　sibiricum　rhizomes　with　multiple　rhizome　buds　(ACM)　was　characterized　by　using　untargeted　UHPLC-Q-Orbitrap-MS　based　approach.　In　addition,　two-wild-types　P.　sibiricum　rhizomes,　namely　the　wild-type　with　multiple　rhizome　buds　(WTM)　and　the　wild-type　with　single　rhizome　bud　(WTS),　were　used　for　comparison.　A　total　of　183　phytochemicals,　including　20　alkaloids,　48　flavonoids,　33　phenolic　acids,　and　82　terpenoids,　were　tentatively　identified.　Generally,　the　phytochemical　profile　of　ACM　was　comparable　to　that　of　WTM　and　WTS.　In　specific,　most　of　the　identified　alkaloids　and　phenolic　acids,　and　approximately　half　of　the　identified　terpenoids,　were　not　significantly　different.　Notably,　several　phytochemicals　with　potent　therapeutic　properties,　such　as　epiberberine,　laetanine,　sinapic　acid,　curcumenol,　were　present　in　ACM.　Additionally,　artificial　cultivation　increased　the　abundance　of　geniposide　and　naringenin,　which　have　been　linked　to　cardioprotective　effects.　These　findings　provide　valuable　insights　for　the　future　utilization　of　artificially　cultivated　P.　sibiricum.