As　a　basic　functional　unit,　living　cell　with　sophisticated　structures　play　an　indispensable　role　in　life　activities.　Since　the　abnormality　of　important　molecules　inside　cells　is　closely　related　to　diseases,　the　dynamic　analysis　and　spatio-temporal　monitoring　of　specific　molecules　in　living　cells　can　provide　precious　information　for　the　diagnosis　and　treatment　of　diseases.　More　recently,　DNA　has　not　only　been　recognized　as　the　carrier　of　genetic　information,　but　has　also　used　as　a　robust　building　block　for　the　assembly　of　multitudinous　nanoscale　structures　due　to　the　intrinsic　advantages　of　high　programmability　of　classic　Watson–Crick　base-pairing　rule.　Intensive　study　promotes　the　rapid　progress　of　nanotechnology　in　various　fields,　such　as　bioimaging,　diagnosis,　and　therapeutics.　Among　numerous　well-defined　DNA　nanomaterials,　DNA　nanomachines　have　been　widely　exploited　in　cell　imaging　owing　to　their　desirable　ability　to　achieve　high-resolution　temporal　and　spatial　images　in　response　to　endogenous　or　exogenous　stimuli.　In　brief,　elaborate　DNA　nanomachines　can　undergo　structural　changes　upon　the　stimuli　of　target　analytes　or　environmental　factors,　resulting　in　rapid　increase　or　reduction　of　output　signals　and　thereby　indirectly　reflecting　the　expression　level　of　targets.　DNA　nanomachines　with　high　sensitivity　and　specificity　contribute　to　the　recognition　of　diseased　tissues.　In　this　review,　we　introduce　the　basic　assembly　modules　of　DNA　nanomachines　and　summarize　the　recent　advances　in　dynamic　DNA　nanomachines　for　diseased-cell　imaging.　Finally,　the　current　challenges　and　future　directions　of　DNA　nanomachines　for　bioimaging　are　discussed.　©　2023　The　Authors