Rice　sheath　blight　(ShB)　caused　by　Rhizoctonia　solani　is　one　of　the　most　serious　diseases　that　threatens　rice　(Oryza　sativa)　production.　However,　the　mechanisms　of　defense　against　ShB　in　rice　remain　largely　unknown.　In　this　study,　we　identified　that　the　expression　levels　of　beta-glucanase　(OsBGL)　family　genes　sensitively　respond　to　infection　by　R.　solani,　and　OsBGLs　positively　regulate　rice　resistance　to　ShB.　In　addition,　OsBGL2　colocalized　with　AtPDCB1　at　the　plasmodesmata　(PD)　and　limited　the　PD　permeability.　The　level　of　callose　accumulation　in　osbgls　mutants　and　overexpressors　was　examined,　and　OsBGLs　were　found　contribute　to　callose　accumulation.　Taken　together,　these　data　suggest　that　OsBGLs　can　regulate　the　deposition　of　callose　at　the　PD　to　reduce　its　permeability　to　defend　itself　against　ShB.　Through　the　identification　of　these　genes　and　the　elucidation　of　their　functions,　this　research　fills　the　gap　in　the　mechanism　of　PD　permeability　in　rice　ShB　resistance.