The　sensitive　detection　of　acetylcholinesterase(AChE)　and　organophosphoruspesticides　(OPs)　is　very　important　for　the　protection　of　human　health.Herein,　a　hybrid　material,　Pt　NPs/Fe-MOF,　consisting　of　a　metal-organicframework　(MIL-88B-NH2,　Fe-MOF)　decorated　with　platinumnanoparticles　(Pt　NPs),　was　prepared　first　and　exhibited　remarkablyimproved　and　excellent　peroxidase-mimicking　activity　compared　to　theFe-MOF　material　resulting　from　the　synergistic　catalysis　effect　betweenFe-MOF　and　Pt　NPs,　which　can　effectively　catalyze　3,3　&　PRIME;,5,5　&　PRIME;-tetramethylbenzidine(TMB)　oxidation　to　generate　a　blue　product　(oxidized　TMB,　oxTMB).Interestingly,　in　the　presence　of　AChE　and　acetylcholinesterase,　theperoxidase-mimicking　activity　from　Pt　NPs/Fe-MOF　was　inhibited　obviously,and　thus,　a　colorimetric　sensing　platform　for　AChE　can　be　constructed;more　importantly,　after　the　addition　of　OPs,　this　nanozyme　activitycan　be　recovered,　inducing　the　further　successful　construction　ofa　sensitive　colorimetric　sensing　platform　for　OPs.　The　related　sensingmechanism　and　condition　optimization　were　studied,　and　the　as-preparedPt　NPs/Fe-MOF　nanozyme-based　colorimetric　method　for　AChE　and　OP　detectiondisplayed　superior　analytical　performances　with　wide　linearities　andlow　detection　limits.　Furthermore,　the　designed　method　offers　satisfactoryreal　application　ability.　We　expect　the　as-proposed　Pt　NPs/Fe-MOFnanozyme-based　colorimetric　sensing　platform　for　AChE　and　OPs　viathe　enzyme　cascade　reaction　to　show　great　potential　application.　A　sensitive　colorimetric　sensing　assayfor　acetylcholinechloride　and　organophosphorus　pesticides　was　fabricated　by　preparinghigh　peroxidase-mimicking　MIL-88B-NH2　MOF　decorated　withplatinum　nanoparticles　as　nanozymes　via　enzyme　cascade　reaction