Bacterial　infection　of　wounds　is　one　of　great　concern　to　patients,　and　rapid　and　correct　detection　of　bacterial　infection　is　crucial　to　ensure　accurate　diagnosis　and　early　intervention.　Based　on　the　principle　that　glucose　can　only　be　consumed　by　live　bacteria,　a　fluorescent　biosensor　was　constructed　to　detect　four　kinds　of　common　infectious　bacteria　(Acinetobacter　baumannii,　Escherichia　coli,　Pseudomonas　aeruginosa　and　Staphylococcus　aureus)　in　skin　wounds,　taking　advantage　of　magnetic　bead-aptamer　for　recognizing,　sorting　and　enrichment,　platinum　nanoparticles　for　signal　amplification.　The　linear　detection　range　of　AB,　EC,　PA　and　SA　were　27–　8　×　106　CFU/mL,　10–　2.5　×　107　CFU/mL,　34–　2.5　×　107　CFU/mL　and　10–　1.0　×　107　CFU/mL,　respectively,　and　the　limits　of　detection　were　27　CFU/mL,　10　CFU/mL,　34　CFU/mL　and　2　CFU/mL.　Furthermore,　all　target　bacteria　in　samples　containing　8　×　108　CFU/mL　of　other　interfering　bacteria　have　been　successfully　identified　and　quantified.　The　proposed　method　was　also　successfully　applied　to　the　detection　of　bacterial　infection　in　skin　wounds　of　mouse　and　human,　including　the　detection　of　separate　bacterial　infection　as　well　as　a　coinfection.　The　recovery　of　this　method　was　in　the　range　of　90.161–　109.961%.　Thus,　this　proposed　method　can　be　a　promising　candidate　for　rapid　and　convenient　evaluation　of　infectious　bacteria　in　point-of-care　settings.　©　2023　Elsevier　B.V.