Combining　the　features　of　the　host-guest　system　and　chirality　is　an　efficient　strategy　to　achieve　circularly　polarized　luminescence　(CPL).　Herein,　well-defined　chiral　carbon　nanodot　(chirCND)　arrays　were　confined-synthesized　by　low-temperature　calcination　of　a　chiral　amino　acid　loaded　metal-organic　framework　(MOF)　to　induce　high　CPL.　An　achiral　porous　pyrene-based　MOF　NU-1000　thin　film　as　the　host　template　was　prepared　by　a　liquid-phase　epitaxial　layer-by-layer　fashion,　and　chiral　amino　acids　as　the　carbon　sources　could　be　confined　in　the　porous　MOF　and　carbonized　to　homogeneous　and　ultrasmall　chirCND　arrays,　resulting　in　a　chirCNDs@NU-1000　thin　film　(L-CNDsx@NU-1000;　x　=　L-cysteine　(cys),　L-serine,　L-histidine,　L-glutamic　acid,　and　L-pyroglutamic　acid).　The　results　show　the　pristine　chirCNDs　by　directly　carbonizing　chiral　amino　acids　hardly　endow　them　with　a　CPL　property.　By　contrast,　benefiting　from　the　arrayed　confinement　and　coordination　interaction　between　chirCNDs　and　NU-1000,　the　chirality　transfer　on　the　excited　state　of　chirCNDs@NU-1000　is　enabled,　leading　to　strong　CPL　performance　(a　high　luminescence　dissymmetry　factor　glum　of　LCNDscys@NU-1000　thin　film　reached　1.74　x　10(-2)).　This　study　of　chirCNDs　encapsulated　in　fluorescent　MOF　thin　films　provides　a　strategy　for　developing　uniform　chiral　carbon　nanoarrays　and　offers　chiral　host-guest　thin-film　materials　for　optical　applications.