Helicobacter　Pylori　infection　is　drawing　increasing　attentions　in　public　health,　especially　the　drug　resistance　problems　induced　by　Single-Nucleotide　Variants　(SNV).　Diagnosis　of　H.　Pylori　remains　challenging　for　its　requirement　in　selectivity　and　sensitivity.　Herein　an　initial　check-reexamination　strategy　is　designed　for　analysis　of　H.　Pylori　DNA　and　SNV.　At　the　first　stage,　target　DNA　with　all　genotypes　is　captured　to　form　a　Y-shaped　structure,　resulting　in　an　electrochemiluminescence　(ECL)　signal　recovered　from　quenched　states.　Then　Cas9　assisted　cleavage　processes　are　followed　to　cut　off　the　Y-shaped　structure,　resulting　in　corresponding　signal　decrease.　By　means　of　these　two　stages　with　different　selectivity,　both　the　total　amount　of　H.　Pylori　DNA　and　the　ratio　of　SNV　can　be　clarified.　To　expand　its　capacity,　a　large-scale　screening　assay　is　carried　out　on　chip.　Array　detection　improves　the　reliability　and　the　following　PCA　analysis　confirms　the　otherness.　This　approach　improved　the　work　efficiency　and　reduced　the　cost,　which　may　offer　an　appealing　option　for　the　prevention　and　cure　of　H.　pylori　infections　in　the　future.　©　2023　Elsevier　B.V.