Ti6Al4V3Cu　alloy　is　a　promising　biomaterial　for　combating　implant-related　infection.　However,　the　antibacterial　property　of　Ti6Al4V3Cu　is　expected　to　be　enhanced　due　to　the　low　content　of　Cu　element　in　titanium.　To　address　this　issue,　the　antibacterial　property　of　Ti6Al4V3Cu　was　tailored　by　the　grooves　with　different　groove　widths　(30 μm,　60 μm,　and　90 μm)　that　were　constructed　on　the　surface　of　the　Ti6Al4V3Cu　by　selective　laser　melting.　The　effect　of　grooves　on　corrosion　resistance,　antibacterial　property,　and　cytocompatibility　was　investigated.　The　electrochemical　tests　showed　that　the　corrosion　resistance　decreased　with　increasing　groove　width.　The　antibacterial　tests　indicated　that　the　groove　with　a　width　of　30 μm　and　90 μm　groups　showed　better　antibacterial　activity　against　S.　aureus　(＞　90%)　compared　with　the　groove　with　a　width　of　60 μm.　The　in　vitro　study　suggested　that　all　samples　with　different　grooves　were　found　to　exhibit　good　cytocompatibility　with　osteoblast　cells.　It　is　considered　that　creating　grooves　on　Ti6Al4V3Cu　by　selective　laser　melting　is　a　promising　strategy　to　enhance　the　antibacterial　activity　without　sacrificing　cytocompatibility.　©　2023,　The　Chinese　Society　for　Metals　(CSM)　and　Springer-Verlag　GmbH　Germany,　part　of　Springer　Nature.